Serotype Kit-CMVeGFP Baculovirus Produced
25ul each of CMVeGFP with the following serotypes: 2/1, 2/2, 2/4, 2/6, 2/8, 2/9.
The Viral Vector Core can produce rAAV via the Baculovirus system (Bac) in Sf9 insect cells from Robert Kotin or in transiently-transfected human HEK293 cells (3X). Vector production platforms have implications for vector binding, expression, trafficking, stability, and immunogenicity due to differences in post translational modifications. We recommend controls from the same system as custom vectors. Order human HEK293 (3X) controls if starting a new project or unsure.
Paulk NK, et al. Methods Matter -- Standard Production Platforms For Recombinant AAV Can Produce Chemically And Functionally Distinct Vectors
Urabe M., Ding C, and Kotin RM. Insect Cells as a Factory to Produce Adeno-Associated Virus. Type 2 Vectors HUMAN GENE THERAPY 13:1935–1943 (November 1, 2002)
Smith RH, Levy JR, Kotin RM. A simplified baculovirus-AAV expression vector system coupled with one-step affinity purification yields high-titer rAAV stocks from insect cells. Mol Ther. 2009;17(11):1888–1896. doi:10.1038/mt.2009.128
Kondratov, O. et al. Direct Head-to-Head Evaluation of Recombinant Adeno-associated Viral Vectors Manufactured in Human versus Insect Cells. Mol. Ther. 25, 2661–2675 (2017).
Giles, A. R. et al. Deamidation of Amino Acids on the Surface of Adeno-Associated Virus Capsids Leads to Charge Heterogeneity and Altered Vector Function. Mol. Ther. (2018). doi:10.1016/j.ymthe.2018.09.013
Ghaderi, D., Zhang, M., Hurtado-Ziola, N. & Varki, A. Production platforms for biotherapeutic glycoproteins. Occurrence, impact, and challenges of non-human sialylation. Biotechnol. Genet. Eng. Rev. 28, 147–175 (2012).
*These listed prices are per aliquot for non-profit customers only, additional fees may apply.